Development and experience of using the transcriptional gene signature in the diagnosis of molecular breast cancer subtypes
Objective. To design and carry out clinical testing a Russian transcriptional gene signature for the diagnosis of molecular breast cancer subtypes.Burmenskaya O.V., Trofimov D.Yu., Kometova V.V., Sergeev I.V., Maerle A.V., Rodionov V.V., Sukhikh G.T.
Subjects and methods. The investigation enrolled 168 women aged 29 to 82 years with breast cancer (BC) who had been treated at the Department of Breast Pathology, Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology. The investigators performed a pathomorphological study of surgical materials and immunohistochemical (IHC) tests for Ki-67, ER, PR, HER2/neu and determined the expression profile of 45 genes of proliferation, apoptosis, invasion, hormone receptors, and growth factor receptors, DNA replication and repair, immunity, transcription factors, and cell differentiation markers by a real-time quantitative polymerase chain reaction assay.
Results. The investigators established the main patterns of mRNA expression of the test genes for various BC subtypes and their correlations, devised and tested methods for the molecular genetic classification of breast tumors as compared with the traditional IHC study.
Conclusion. The developed transcriptional gene signature reflects the heterogeneity of breast tumors and can be used for their molecular genetic classification.
Keywords
breast cancer
tumor heterogeneity
molecular breast cancer subtypes
transcriptional gene signatures
real-time PCR
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Received 19.09.2019
Accepted 04.10.2019
About the Authors
Olga V. Burmenskaya, SD, Head of the Laboratory of Oncological Genetics, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov, Ministry of Heals of Russia. Tel.: +7 (495) 438-22-92. E-mail: o_bourmenskaya@oparina4.ru117997, Russia, Moscow, Ac. Oparina, St. 4.
Dmitry Yu. Trofimov, professor, SD, Director of the Institute of Reproductive Genetics National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov, Ministry of Heals of Russia. Tel.: +7(495)438-49-51. Е-mail: d_trofimov@oparina4.ru
117997, Russia, Moscow, Ac. Oparina, St. 4.
Vlada V. Kometova, MD, Senior Researcher, Pathology Department, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov, Ministry of Heals of Russia. Tel.: +7(495) 438-23-11 Е-mail: v_kometova@oparina4.ru
117997, Russia, Moscow, Ac. Oparina, St. 4.
Ilia V. Sergeev, PhD, Head of Oligonucleotide synthesis department «NPF DNA-Technology» Ltd. Tel: +7(495)640-17-71. Е-mail: i.sergeev@dna-technology.ru
117246, Russia, Moscow, Nauchnyy passage, 20/4.
Artem V. Маеrle, Head of QC unit of Oligonucleotide synthesis department «NPF DNA-Technology» Ltd. Tel.: +7(495)640-17-71. Е-mail:a.maerle@dna-technology.ru
117246, Russia, Moscow, Nauchnyy passage, 20/4.
Valery V. Rodionov, MD, professor, Head of the Department of Breast Cancer Department, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov, Ministry of Heals of Russia. Tel.: +7(926)629-34-00. Е-mail: v_rodionov@oparina4.ru
117997, Russia, Moscow, Ac. Oparina St., 4.
Genady T. Sukhikh, MD, Academician RAS, professor, Director of National Medical Research Center of Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov, Ministry of Heals of Russia. Тel.: +7(495)438-18-00 Е-mail: g_sukhikh@oparina4.ru
117997, Russia, Moscow, Ac. Oparina St., 4.
For citation: Burmenskaya O.V., Trofimov D.Yu., Kometova V.V., Sergeev I.V., Maerle A.V., Rodionov V.V., Sukhikh G.T. Development and experience of using the transcriptional gene signature in the diagnosis of molecular breast cancer subtypes.
Akusherstvo i Ginekologiya/ Obstetrics and gynecology. 2020;2: 132-40.(In Russian).
https://dx.doi.org/10.18565/aig.2020.2.132-140
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