Development of the bioartificial vaginal wall: an in vitro stage
Objective. To obtain a tissue-engineered construct based on a nonwoven polycaprolactone carrier, human epithelial and stromal cells to make an artificial vagina.Arutyunyan I.V., Tenchurin T.Kh., Grigoryev T.E., Makarov A.V., Korshunov A.A., Elchaninov A.V., Kananykhina E.Yu., Lokhonina A.V., Vasyukova O.A., Uvarova E.V., Chuprynin V.D., Shepelev A.D., Mamagulashvili V.G., Chvalun S.N., Fatkhudinov T.Kh.
Material and methods. Fibrous material based on polycaprolactone was obtained by an electrospinning technique. A capillary method was used to colonize the full-thickness tissue-engineering design by stromal cells and a static method was employed to colonize the surface layer of the construct by vaginal epithelial cells.
Results. The vaginal epithelial cells expressed EpSAM and p63; the stromal cells did vimentin and smooth muscle actin. After colonization, the stromal cells were evenly distributed in a 1.5-mm thick matrix; the epithelial cells were arranged in a dense layer on the inner surface of the construct, sinking to a depth of 88.9±32.5 µm.
Conclusion. The tissue-engineered construct obtained is similar to the native vagina in architectonics and cell composition.
Keywords
vagina
tissue engineering
polycaprolactone matrix
electrospinning
vaginal epithelium
multipotent stromal cells
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Received 25.08.2017
Accepted 22.09.2017
About the Authors
Arutyunyan Irina Vladimirovna, PhD, Researcher, Laboratory of Regenerative Medicine, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; researcher of the Laboratory of Growth and Development of Research Institute of Human Morphology.117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79261474430. E-mail: labrosta@yandex.ru. ORCID iD 0000-0002-4344-8943
Tenchurin Timur Khasyanovich, Ph.D., Research Fellow, Laboratory of Polymer Materials, Kurchatov Complex of Nano-, Bio-, Information, Cognitive,
and Sociohumanistic Technologies, National Research Center, Kurchatov Institute.
123182, Russia, Moscow, Academician Kurchatov sq. 1. Tel.: +79265283237. E-mail: tenchurin.timur@mail.ru. ORCID iD 0000-0003-1000-6003
Grigoryev Timofey Evgenievich, Ph.D., Head of the Department of Nanobiomaterials and Structures of Kurchatov Complex of Nano-, Bio-, Information, Cognitive,
and Sociohumanistic Technologies, National Research Center, Kurchatov Institute.
123182, Russia, Moscow, Academician Kurchatov sq. 1. Tel.: +79265602000. E-mail: timgrigo@yandex.ru.
Makarov Andrey Vitalevich, Candidate of Medical Sciences, Senior Researcher Laboratory of Regenerative Medicine, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; associate professor of the Department of Histology, N.I. Pirogov Russian National Research Medical University. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79032563404. E-mail: anvitmak@yandex.ru. ORCID iD 0000-0003-2133-2293
Korshunov Alexey Alexandrovich, obstetrician-gynecologist of the department of combined and complex methods of treatment of gynecological diseases, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954380988. E-mail: a_korshunov@oparina4.ru
Yelchaninov Andrei Vladimirovich, Candidate of Medical Science, Senior Researcher Laboratory of Regenerative Medicine, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia, Senior Researcher Laboratory of growth and development of Research Institute of Human Morphology. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79168885292. E-mail: elchandrey@yandex.ru. ORCID iD 0000-0002-2392-4439
Kananyhina Yevgenia Yurievna, m.n. Laboratory of Regenerative Medicine, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; graduate student of Research Institute of Human Morphology.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79258842800. E-mail: e.kananykhina@gmail.com. ORCID iD 0000-0002-9779-2918
Lokhonina Anastasia Vyacheslavovna, m.n. Laboratory of Regenerative Medicine, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79104316676. E-mail: nastya.serbsky@gmail.com. Orcid ID: 0000-0001-8077-2307
Vasyukova Olesya Alexandrovna, the executor of the Agreement with the Ministry of Education and Science of the Russian Federation 14.613.21.0008 from 08/22/2014 National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia;
m.n. Laboratory of growth and development of Research Institute of Human Morphology.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79639972679. E-mail: olesya.vasyukova.rnrmu@gmail.com. ORCID iD 0000-0001-6068-7009
Uvarova Elena Vitalevna, MD, professor, head. 2-nd gynecological department (gynecology of children and adolescents) National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; President of the Interregional Public Organization “
Association of Children and Adolescent Gynecologists”.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954388542. E-mail: elena-uvarova@yandex.ru
Chuprinin Vladimir Dmitrievich, Ph.D., head. surgical department of National Medical Research Center of Obstetrics, Gynecology, and Perinatology named
after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954388508. E-mail: v_chuprinin@oparina4.ru
Shepelev Alexey Dmitrievich, Ph.D., engineer-researcher of the laboratory of biocompatible matrices of Kurchatov Complex of Nano-, Bio-, Information, Cognitive,
and Sociohumanistic Technologies, National Research Center, Kurchatov Institute.
123182, Russia, Moscow, Academician Kurchatov sq. 1. Tel.: +79163166215. E-mail: shepelev_alexey@mail.ru. ORCID iD 0000-0003-1997-967X.
Mamagulashvili Vissarion Georgievich, Ph.D., engineer-researcher of the laboratory of biocompatible matrices of Kurchatov Complex of Nano-, Bio-, I
nformation, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute.
123182, Russia, Moscow, Academician Kurchatov sq. 1. Tel.: +79629158954. E-mail: beso52@mail.ru. ORCID iD 0000-0003-1439-1535.
Chvalun Sergey Nikolaevich, Doctor of chemical sciences, professor, deputy director for scientific work of Kurchatov Complex of Nano-, Bio-, Information, Cognitive, and Sociohumanistic Technologies, National Research Center, Kurchatov Institute.
123182, Russia, Moscow, Academician Kurchatov sq. 1. Tel.: +79166102908. E-mail: s-chvalun@yandex.ru.
Fatkhudinov Timur Khaisamudinovich, MD, Ph.D. Laboratory of Regenerative Medicine, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia; Professor of the Department of Histology, Cytology and Embryology of Peoples’ Friendship University of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79032561157. E-mail: tfat@yandex.ru. ORCID iD 0000-0002-6498-5764.
For citations: Arutyunyan I.V., Tenchurin T.Kh., Grigoryev T.E., Makarov A.V.,
Korshunov A.A., Elchaninov A.V., Kananykhina E.Yu., Lokhonina A.V., Vasyukova O.A., Uvarova E.V., Chuprynin V.D., Shepelev A.D., Mamagulashvili V.G., Chvalun S.N.,
Fatkhudinov T.Kh. Development of the bioartificial vaginal wall: an in vitro stage.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (5): 70-7. (in Russian)
https://dx.doi.org/10.18565/aig.2018.5.70-77
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