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The Journal "Obstetrics and Gynecology"Journal HistoryAims and ScopePolicies on Conflict of Interest, Human and Animal Rights, and Informed ConsentEditorial ProcessData sharing statementAdvertising PolicyThe Process for Handling Cases Requiring Corrections, Retractions, and Editorial Expressions of ConcernEditorial BoardEditorial CounsilInformation for ProfessionalsNewsContacts
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Obstetrics and Gynegology2024№11
Method of laser conversion testing...

Method of laser conversion testing for diagnosing the functional state of endometrium

DOI
https://dx.doi.org/10.18565/aig.2024.264

Dikke G.B., Sukhanov A.A., Kukarskaya I.I., Shilova N.V.

1) F.I. Inozemtsev Academy of Medical Education, St. Petersburg, Russia; 2) Tyumen State Medical University, Ministry of Health of Russia, Tyumen, Russia; 3) Perinatal Medical Center, Tyumen, Russia; 4) Gen-Lab LLC, Moscow, Russia

Background: The search for informative methods to determine the readiness of the endometrium for embryo implantation in patients with infertility caused by chronic endometritis (CE) represents a significant issue in the field of reproductive medicine.
Objective: To evaluate the possibility of using the technology of Laser Conversion Testing (LCT) performed with the PHOTON-BIO spectrometer for diagnosing the functional state of endometrium and determining its readiness for embryo implantation in patients with uterine infertility caused by CE.
Materials and methods: The study was conducted in two stages. Stage I involved the evaluation of the LCT method for the diagnosis of CE and the functional activity of the endometrium (n=40). Stage II aimed to verify the performance of the LCT method among patients with CE and infertility during preconception preparation prior to IVF (n=300). The following methods were used: pelvic ultrasound, Dopplerometry, histological and immunohistochemical studies of endometrial biopsy specimens, and LCT with the PHOTON-BIO spectrometer.
Results: The LCT method was evaluated for reproducibility (Cohen’s kappa coefficient – from 0.59 to 1.0), precision (variability) of results, analytical (diagnostic) characteristics (sensitivity 61.4–81.2%, specificity 76.8–89.7%, positive predictive value 50–59.8%, negative predictive value 53.9–97.8%) and accuracy (63.3–69.7%). The results showed its feasibility and scientific validity for solving a specific problem, namely, improving reproductive outcomes by preventing failed embryo transfers in assisted reproductive technology programs. The use of LCT for this purpose makes it possible to significantly improve the results of IVF and achieve pregnancy in 83.8% and live birth in 72.1% of cases among the patients who underwent several courses of treatment with a complex of antimicrobial peptides and cytokines; the amount of these substances was determined individually using this method.
Conclusion: The LCT medical technology meets the objectives of diagnosing the functional state of the endometrium in patients with CE and is effective in determining the readiness of the endometrium for embryo implantation. 

Authors’ contributions: Dikke G.B. – developing the concept, design and program of the study, analysis of the results of statistical processing of clinical material and their interpretation, search of literature sources, writing the article and its editing after review, approval of the final version of the article; Sukhanov A.A. – collecting clinical material, formation of the electronic database, writing fragments of the article and its editing after review, approval of the final version of the article; Kukarskaya I.I. – organization of the study at the clinical base, guidance during the study, approval of the final version of the article; Shilova N.V. – preparation of the electronic database for analysis, statistical analysis of the study results, design of tables, writing fragments of the article, approval of the final version of the article.
Conflicts of interest: The authors declare no possible conflicts of interest. 
Funding: The study was not sponsored.
Patient Consent for Publication: The patient signed an informed consent for the publication of her data and impersonal images.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Dikke G.B., Sukhanov A.A., Kukarskaya I.I., Shilova N.V. Method of 
laser conversion testing for diagnosing the functional state of endometrium.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2023; (11): 186-198 (in Russian)
https://dx.doi.org/10.18565/aig.2024.264

Keywords

chronic endometritis
reproductive function
infertility
laser conversion testing
in vitro fertilisation
immunomodulators
antimicrobial peptides and cytokines
Superlymph
Full text (in Russian)

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Received 22.10.2024

Accepted 31.10.2024

About the Authors

Galina B. Dikke, Dr. Med. Sci., Professor at the Department of Obstetrics and Gynecology with a course in Reproductive Medicine, F.I. Inozemtsev Academy of Medical Education, 190013, Russia, St. Petersburg, Moskovsky Ave., 22, letter M, galadikke@yandex.ru, https://orcid.org/0000-0001-9524-8962
Anton A. Sukhanov, PhD, Head of the Department of Gynecology, Perinatal Center, 625002, Russia, Tyumen, Daudelnaya str., 1; Associate Professor at the Department of Obstetrics and Gynecology, Institute of Maternity and Childhood, Tyumen State Medical University, Ministry of Health of Russia, 625023, Russia, Tyumen, Odesskaya str., 54, such-anton@yandex.ru, https://orcid.org/0000-0001-9092-9136
Irina I. Kukarskaya, Dr. Med. Sci., Associate Professor, Head of the Department of Obstetrics, Gynecology and Perinatology, Institute of Motherhood and Childhood,
Tyumen State Medical University, Ministry of Health of Russia, 625023, Russia, Tyumen, Odesskaya str., 54; Chief Physician, Perinatal Center, 625002, Russia, Tyumen, Daudelnaya str., 1; Chief Specialist in Obstetrics and Gynecology, Department of Health of the Tyumen Region, kukarskay@mail.ru, https://orcid.org/0000-0002-8275-3553
Natalya V. Shilova, PhD, Medical Director, Gen-Lab, 107078, Russia, Moscow, Kalanchevskaya str., 31-4, nvshilova@gmail.com, https://orcid.org/0000-0001-6734-0147
Corresponding author: Galina B. Dikke, galadikke@yandex.ru

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