Investigation of the gene mRNA level in the endometrial tissue of reproductive-aged women with intrauterine synechiae
Objective. To identify molecular genetic markers of the formation of intrauterine synechiae to improve management tactics in reproductive-aged women.Khirieva P.M., Kuznetsova M.V., Bystritsky A.A., Martynov S.A., Burmenskaya O.V., Trofimov D.Yu., Adamyan L.V.
Subjects and methods. A total of 20 patients who had been operated on at the Department of Gynecology,
V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, were examine during a prospective study. The patients were divided into 2 groups: 1) those with intrauterine synechiae (a main group); 2) apparently healthy women (a comparison group). Endometrial tissue samples were preoperatively taken using pipelle biopsy, then the expression profile was obtained by microarray hybridization with the GeneChip Human Exon 1.0 ST Arrays (Affymetrix, USA) according to the manufacturer’s protocol. Bioinformatic analysis of the data obtained by micrometrical analysis was carried out using the Gene Ontology service.
Results. The endometrium of women with intrauterine synechiae showed a higher mRNA level in 9 genes and a lower mRNA one in 2 genes than that in the comparison group. The bioinformatic analysis revealed that 6 of the 9 genes were involved in immune response, including neutrophil activation, 4 of the 9 genes were in exocytosis.
Conclusion. The involvement of most detected genes in the processes of immune response, inflammation, apoptosis, and exocytosis suggests that there is an abnormal cell-cell interaction in patients with intrauterine synechiae. The change in the level of mRNA in the S100A8, HBB, VNN2, RGS2, ERAP2, AQP9, MNDA, TUBA3E, and FSGR3B genes in the endometrium of women with intrauterine synechiae may serve as a basis for the creation of test systems to detect mRNA by RT-PCR technique as a minimally invasive test used in clinical practice to optimize management tactics in reproductive-aged women with intrauterine synechiae in order to enhance the effectiveness of treatment outcomes.
Keywords
intrauterine synechiae
genes
microarrays
endometrial fibrosis
Supplementary Materials
- Table 1. Expression of genes in the endometrium in the proliferative phase in women with intrauterine adhesions compared with the endometrium of conditionally healthy women
- Table 2. Results of bioinformatics analysis of data obtained using micrometrical analysis
- Figure. Hierarchical cluster analysis comparing gene expression in the endometrium of women with intrauterine adhesions and conditionally healthy women
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Received 08.09.2017
Accepted 22.09.2017
About the Authors
Khirieva Patimat Magomedovna, postgraduate student of gynecological department, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387783. E-mail: dr.khirieva@rambler.ruKuznetsova Maria Vladimirovna, PhD, Researcher of the laboratory of molecular genetics methods, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: mkarja@mail.ru
Bystritskiy Andrei Aleksandrovich, PhD, Leading Researcher of the laboratory of molecular genetics methods, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: andrey.bystritskiy@yandex.ru
Martynov Sergey Aleksandrovich, MD, Leading Researcher of gynecological department, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387783. E-mail: s_martynov@oparina4.ru
Bourmenskaya Olga Vladimirovna, MD, Researcher of the laboratory of molecular genetics methods, National Medical Research Center of Obstetrics, Gynecology,
and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: o_bourmenskaya@oparina4.ru
Trofimov Dmitrii Yurievich, MD, Head of the laboratory of molecular genetics methods, National Medical Research Center of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954384951. E-mail: d_trofimov@oparina4.ru
Adamyan Leyla Vladimirovna, MD, professor, Academician of RAS, Deputy Director for Science, Head of gynecological department, National Medical Research Center
of Obstetrics, Gynecology, and Perinatology named after Academician V.I. Kulakov, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387783. E-mail: l_adamyan@oparina4.ru
For citations: Khirieva P.M., Kuznetsova M.V., Bystritsky A.A., Martynov S.A., Burmenskaya O.V., Trofimov D.Yu., Adamyan L.V. Investigation of the gene mRNA level in the endometrial tissue of reproductive-aged women with intrauterine synechiae. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2018; (2): 56-64. (in Russian)
https://dx.doi.org/10.18565/aig.2018.2.56-64
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