Study of inflammatory and angiogenic biomarker levels in various biological fluids in women with extragenital endometriosis
Kudryavtseva E.V., Mangileva Ya.A., Polushina L.G., Maksimova A.Yu., Kopenkin M.A., Zornikov D.L., Kovalev V.V.
Objective: To analyze the content of biomarkers of inflammation and angiogenesis in oral and peritoneal fluids in women with a common form of extragenital endometriosis including ovarian lesions.
Materials and methods: This was a comparative observational study. Group 1 (n=88) consisted of women with confirmed extragenital endometriosis including endometrioid ovarian cysts; group 2 (n=30) consisted of healthy women without signs of endometriosis. The samples of oral fluid (OF) were collected from all participants of the study, and samples of peritoneal fluid (PF) were collected from women of group 1. The levels of vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNF-α), and interleukins (IL), namely IL-1β, IL-6, IL-8, and IL-10 were studied in biological fluids.
Results: The level of VEGF in OF was significantly higher in the patients of group 1, namely 906.4 (281.9–178) pg/ml compared to the patients of group 2, 241.9 (105.225–324.475) pg/ml, p<0.001. The level of IL-8 was also significantly higher in group 1 compared to group 2, 100.85 (23.333–208.7) pg/ml versus 11.34 (6.549–21.205) pg/ml, respectively, p<0.001. The levels of TNF-α, IL-6, and IL-10 were higher in group 2. The analysis of the correlation between the levels of markers in OF and PF showed no correlation for IL-8, IL-10 and TNF-α and the presence of a very weak, nonlinear relationship for IL-1β, IL-6 and VEGF.
Conclusion: The data obtained suggest the independence of inflammatory and angiogenic biomarker profiles in different biological compartments. The results highlight the potential for future research into OF as a potential bio substrate that could indirectly reflect the characteristics of the systemic inflammatory response in patients with extragenital endometriosis.
Authors’ contributions: Kudryavtseva E.V., Kovalev V.V. – developing the concept and design of the study; Mangileva Ya.A., Polushina L.G., Kopenkin M.A., Maksimova A.Yu. – collecting and processing the material; Kudryavtseva E.V., Zornikov D.L. – statistical processing of the data; Kudryavtseva E.V., Mangileva Ya.A. – writing the text; Zornikov D.L., Kovalev V.V. – editing the article.
Conflicts of interest: The authors declare no possible conflicts of interest.
Funding: The study was conducted without sponsorship.
Ethical Approval: The study was approved by the Ethical Review Board of the Ural State Medical University, Ministry of Health of Russia (Protocol No.11 dated 24/12/2021).
Patient Consent for Publication: The patients signed informed consent for the publication of their data.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Kudryavtseva E.V., Mangileva Ya.A., Polushina L.G., Maksimova A.Yu., Kopenkin M.A.,
Zornikov D.L., Kovalev V.V. Study of inflammatory and angiogenic biomarker levels in various
biological fluids in women with extragenital endometriosis.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2025; (7): 112-120 (in Russian)
https://dx.doi.org/10.18565/aig.2025.116
Keywords
endometriosis
oral fluid analysis
interleukin
laparoscopy
ovarian cyst
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Received 30.04.2025
Accepted 18.06.2025
About the Authors
Elena V. Kudryavtseva, Dr. Med. Sci., Associate Professor, Professor at the Department of Obstetrics and Gynecology, A.B. Blokhin Ural Institute of Healthcare Management, 620075, Russia, Yekaterinburg, K. Liebknecht str., 8-B, elenavladpopova@yandex.ru, https://orcid.org/0000-0003-2797-1926Yana A. Mangileva, Obstetrician-Gynecologist, UGMK-Health LLC, 620144, Russia, Yekaterinburg, Sheinkman str., 113, bld. 2, yanaamangileva@mail.ru,
https://orcid.org/0000-0001-5693-0264
Larisa G. Polushina, PhD, Senior Researcher at the Central Research Laboratory, Ural State Medical University, Ministry of Health of Russia, 620024, Russia, Yekaterinburg, Repin str., 3, polushina-larisa@bk.ru, https://orcid.org/0000-0002-4921-7222
Arina Yu. Maksimova, PhD, Researcher at the Central Research Laboratory, Ural State Medical University, Ministry of Health of Russia, 620024, Russia, Yekaterinburg,
Repin str., 3, oreshek92@list.ru, https://orcid.org/0000-0001-8412-4315
Maxim A. Kopenkin, Junior Researcher at the Central Research Laboratory, Ural State Medical University, Ministry of Health of Russia, 620024, Russia, Yekaterinburg,
Repin str., 3, maximkopenkin@yandex.ru, https://orcid.org/0000-0002-6092-3734
Danila L. Zornikov, PhD, Associate Professor, Department of Medical Microbiology and Clinical Laboratory Diagnostics, Ural State Medical University, Ministry of Health
of Russia, 620024, Russia, Yekaterinburg, Repin str., 3, zornikovdl@yandex.ru, https://orcid.org/0000-0001-9132-215X
Vladislav V. Kovalev, Dr. Med. Sci., Professor, Head of the Department of Obstetrics and Gynecology, A.B. Blokhin Ural Institute of Healthcare Management,
620075, Russia, Yekaterinburg, K. Liebknecht str., 8-B, vvkovalev55@gmail.com, https://orcid.org/0000-0001-8640-8418
Corresponding author: Elena V. Kudryavtseva, elenavladpopova@yandex.ru
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