Use of transcriptomic databases for the analysis of pathogenetic factors of endometriosis
Background. Molecular mechanisms in the pathogenesis of endometriosis remain inadequately studied, largely determining the lack of highly efficient therapy for this disease. Omix technologies used in the study of endometriosis have recently led to the accumulation of information on a change in the quantity and composition of different classes of molecules in intact and pathologically changed tissues.Bobrov M.Yu., I. Balashov S., Filippova E.S., Almova I.K., Khilkevich E.G., Pavlovich S.V., Naumov V.A., Borovikov P.I., Sukhikh G.T.
Objective. To analyze data sets obtained in different studies.
Subject and methods. Six data sets for mRNA expression and nine ones for miRNA expression in the atopic and ectopic endometrium were analyzed. 79 genes with unidirectional expression changes were selected in most collections and functionally characterized.
Results. Comparison of the lists of differentially expressed genes and microRNAs and evaluation of their possible interactions could identify nine miRNAs that are able to be involved in the regulation of expression of ten genes. Analysis of the processes regulated by the differentially expressed genes revealed a number of cellular functions and intracellular signaling pathways that which have not previously been indicated in endometriosis.
Conclusion. Dysregulation associated with these pathways can make a substantial contribution to the development of this disease.
Keywords
endometriosis
path of intracellular signaling
pathogenesis
microRNA expression
„omix” technologies
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Received 07.02.2017
Accepted 17.02.201
About the Authors
Bobrov Mikhail Yu., Ph.D., Head of the Laboratory of Molecular Pathophysiology, Research Center of Obstetrics, Gynecology and Perinatology,Ministry of Health of Russia; Senior Researcher, Laboratory of Oxylipins, Academicians M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry.
117997, Russia, Moscow, Ac. Oparina str. 4
Balashov Ivan S., Ph.D., Laboratory of Bioinformatics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4
Borovikov Pavel I., head of the Laboratory of Bioinformatics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4
Naumov Vladimir, Ph.D., Laboratory of Bioinformatics, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4
Almova Indira K., postgraduate student of the Surgical Department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4
Khilkevich Elena Grigorevna, leading researcher of General Surgery, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina Str. 4. Tel.: +74954387783. E-mail: e_khilkevich@oparina4.ru
Pavlovich Stanislav V., MD, PhD, Scientific secretary, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381800. E-mail: s_pavlovich@oparina4.ru
Fillipova Elena Sergeevna, post-graduate of the gynecological department, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Ac. Oparina str. 4
Sukhikh Gennadiy Tikhonovich, Academician of RAS, MD, PhD, Professor, Director, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381800. E-mail: g_sukhikh@oparina4.ru
For citations: Bobrov M.Yu., I. Balashov S., Filippova E.S., Almova I.K., Khilkevich E.G., Pavlovich S.V., Naumov V.A., Borovikov P.I., Sukhikh G.T. Use of transcriptomic databases for the analysis of pathogenetic factors of endometriosis. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (4): 34-44. (in Russian)
http://dx.doi.org/10.18565/aig.2017.4.34-44
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