Chemomicrobiomic analysis of myoinositol, D-chiroinositol, folic acid and pyroglutamate anion in the nutritional support of female reproductive system

Torshin I.Yu., Gromov A.N., Gromova O.A.

Federal Research Center “Computer Science and Control” of the Russian Academy of Sciences, Moscow, Russia
Background: Myoinositol (MI) and D-chiroinositol (DCI) are used in the treatment of female reproductive disorders and may support the growth of beneficial microbiota.
Objective: To conduct a comparative chemomicrobiomic analysis of MI, DCI and their synergists, namely folic acid (FA) and manganese pyroglutamate (PM) taken orally.
Materials and methods: Chemomicrobiomic analysis included assessments of the accumulation of molecules in various tissues, the area under the growth curve of commensal bacteria (AUC), effects on metabolic microbiota, and minimum inhibitory concentrations (MIC) for pathogenic bacterial strains.
Results: MI, DCI, FA and PM were found to have a synergistic effect on the human microbiome. The greatest contribution to the support of the beneficial microbiota was made by MI (AUC 0.74±0.17 c.u.), followed by DHI and FC (AUC ranged from 0.68 to 0.71 c.u.). Normobiota most actively process FA (19.3±10.5%) and DCI (15.3±11.5%), while MI (13.7±7.7%) and PM (13.2±11.3%) are processed least actively. The most active bacteria metabolizers of the above substances were various strains of Bacteroides fragilis (28–34%). According to the results of chemomicrobiomic analysis, MI, DCI, FA, and PM can exhibit prebiotic properties and support the growth of commensals from the genera Bacteroides, Streptococci, and Bifidobacteria. Since MI, DHI, FA and PM have prebiotic properties, they can support the growth of the normal microflora which is important for the regulation of carbohydrate metabolism. The above substances (especially DHI) can inhibit the growth of pathogenic microorganisms, namely Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae (MIC values are about 20–30 µg/ml).
Conclusion: The analysis made it possible to establish that the combined intake of MI, DCI, FA and PM contributes to the maintenance of a healthy microbiome which is important for a woman’s health, especially during pregnancy and perimenopause.

Author’s contributions: Torshin I.Yu., Gromov A.N., Gromova O.A.: developing the concept of the study, collecting and processing the material, writing the text, editing the article.
Conflicts of interest: The authors declare no possible conflicts of interest.
Funding: The study was conducted without sponsorship.
Authors' Data Sharing Statement: The data supporting the findings of this study are available on request from the corresponding author after approval from the principal investigator.
For citation: Torshin I.Yu., Gromov A.N., Gromova O.A. Chemomicrobiomic analysis of myoinositol, D-chiroinositol, folic acid and pyroglutamate anion in the nutritional support of female reproductive system.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2023; (8): 154-164 (in Russian)


manganese pyroglutamate
area under the growth curve
data mining


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Received 10.08.2023

Accepted 21.08.2023

About the Authors

Ivan Yu. Torshin, PhD in Applied Mathematics, Institute of Pharmacoinformatics, Senior Researcher at the Department of Intellectual Systems, Federal Research Center “Computer Science and Control” of Russian Academy of Sciences,, 119333, Russia, Moscow, Vavilova str., 42.
Andrey N. Gromov, research engineer, Federal Research Center “Computer Science and Control” of Russian Academy of Sciences, SPIN code: 8034-7910,
AuthorID: 15082, WOS ID: C-7476-2018, ResearcherID: C-7476-2018, Scopus Author ID: 7102053964,, ​
119333, Russia, Moscow, Vavilova str., 42.
Olga A. Gromova, Dr. Med. Sci., Professor, Science Нead of the Institute of Pharmacoinformatics, Leading Researcher at the Department of Intellectual Systems,
Federal Research Center “Computer Science and Control” of Russian Academy of Sciences,,,
119333, Russia, Moscow, Vavilova str., 42.

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