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The Journal "Obstetrics and Gynecology"Journal HistoryAims and ScopePolicies on Conflict of Interest, Human and Animal Rights, and Informed ConsentEditorial ProcessData sharing statementAdvertising PolicyThe Process for Handling Cases Requiring Corrections, Retractions, and Editorial Expressions of ConcernEditorial BoardEditorial CounsilInformation for ProfessionalsNewsContacts
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Obstetrics and Gynegology2022№6
Evaluation of extracellular DNA as...

Evaluation of extracellular DNA as a method for non-invasive preimplantation genetic testing of embryos in infertility treatment programs using assisted reproductive technologies

DOI
https://dx.doi.org/10.18565/aig.2022.6.13-19

Lisitsyna O.I., Makarova N.P., Dolgushina N.V.

Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia, Moscow, Russia
A number of factors, the key one of which is the quality of the embryo transferred into the uterine cavity, influence the effectiveness of assisted reproductive technology programs. The embryo quality is most commonly assessed on the basis of morphological criteria and the results of preimplantation genetic testing for aneuploidy (PGT-A). However, these methods have a number of limitations. The detection of extracellular genomic and mitochondrial DNA in the used culture medium has opened up new prospects for the development of non-invasive diagnostic methods in reproductive medicine. To date, a number of scientific papers have already traced the possible implementation of non-invasive PGT-A (niPGT-A). The frequency of correspondence between the results obtained from the analysis of extracellular DNA (ecDNA) of the used culture medium and the results of the assessment of embryonic DNA has been assessed in the study of polar bodies, trophectoderm cells, and the entire embryo. This review discusses in detail the diagnostic possibilities of using niPGT-A methods in the spent culture medium, as well as the effectiveness and future prospects for their use in clinical practice. Particular attention is paid to the technical aspects of the embryological and laboratory stages. The main areas for further full study of the problem are outlined.
Conclusion: The new non-invasive approach to assessing the quality of embryos will be able to more efficiently select embryos with a high potential for further development, ongoing pregnancy and the birth of a healthy baby. The above procedure will allow more patients to receive the necessary medical care, the time to pregnancy to be reduced, and the diagnostic process to be also made more cost-effective.

Keywords

extracellular DNA
genomic DNA
non-invasive preimplantation genetic testing for aneuploidy (n-PGT-A)
used culture medium
Full text (in Russian)

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Received 15.03.2022

Accepted 01.06.2022

About the Authors

Olga I. Lisitsyna, postgraduate student, Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health
of the Russian Federation, o_yazykova@inbox.ru, https://orcid.org/0000-0002-7775-3508, 4, Oparina str., Moscow, 117997, Russia.
Nataliya P. Makarova, Dr. Bio. Sci., Leading Researcher of IVF Department, Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, np_makarova@oparina4.ru, https://orcid.org/0000-0003-1396-7272, 4, Oparina str., Moscow, 117997, Russia.
Nataliya V. Dolgushina, Dr. Med. Sci., Professor, Deputy Director – Head of the Department of Research Administration, Academician V.I. Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of the Russian Federation, n_dolgushina@oparina4.ru,
https://orcid.org/0000-0003-1116-138X, 4, Oparina str., Moscow, 117997, Russia.

Authors' contributions: Lisitsyna O.I., Makarova N.P., Dolgushina N.V.: concept and design of the investigation, writing and editing the text.
Conflicts of interest: The authors declare that there are no conflicts of interest.
Funding: The investigation has not been conducted without attracting additional funding from third parties.
For citation: Lisitsyna O.I., Makarova N.P., Dolgushina N.V. Evaluation of extracellular
DNA as a method for non-invasive preimplantation genetic testing of
embryos in infertility treatment programs using assisted reproductive technologies.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2022; 6: 13-19 (in Russian)
https://dx.doi.org/10.18565/aig.2022.6.13-19

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