Certain acute-phase proteins and cytokines in the serum and follicular liquid of infertile patients with grades 1–2 external genital endometriosis
Objective. To study the levels of certain acute-phase proteins (α2-macroglobulin (α2-MG), α1-antitrypsin (α1-AT)); lactoferrin (LF) and albumin (ALB), cytokines (tumor necrosis factor-α (TNF-α); interleukin-6 and 8 (IL-6 and IL-8), interferon-γ (IFN-γ), as well as total protein (TP) in the infertile patients’ serum and follicular fluid as possible diagnostic markers for grades 1-2 external genital endometriosis (EGE), as well as in the prognosis of in vitro fertilization (IVF).Likhacheva V.V., Krasnopolskaya K.V., Bazhenova L.G., Zorina R.M., Markdorf A.G., Renge L.V., Tretyakova T.V., Shramko S.V.
Subjects and methods. Examinations were made in 32 infertile patients with grades 1-2 EGE (a study group) and in 37 healthy women with male factor infertility (a control group) who had undergone IVF treatment for fertility recovery in the Medica-2 Clinic, “Mother and Child” Group of Companies, Novokuznetsk (Russia). The investigators measured the levels of α2-MG and α1-АТ by quantitative rocket immunoelectrophoresis; LF and cytokines by enzyme immunoassay; ALB and TP by biochemical assays (based on bromocresol green and biuret reagents, respectively). The results obtained were statistically analyzed in a free software environment for statistical data processing using the R version 3.4.
Results. The infertile patients with grades 1-2 ЕGE versus the women in the control group were noted to have elevated serum levels of acute-phase proteins (α2-MG, α1-AT, and ALB), TP, and IFN-γ. The specific feature of the composition of follicular fluid in this patient group was an increase in the concentration of IFN-γ and a decrease in LF and IL-8. The serum ALB concentration above 47.5 g/l on the day of follicle puncture in patients with grades 1-2 EGE is an unfavorable background for the occurrence of pregnancy and is associated with the negative outcome of the IVF program.
Conclusion. The increased concentrations of acute-phase proteins and cytokines (α2-MG, α1-АТ, and IFN-γ), and TP in the blood of infertile women with grades 1-2 EGE can serve as additional diagnostic markers of the disease. The serum albumin level above 47.5 g/l on the day of follicle puncture was associated with the negative outcome of the IVF program in 80% of patients with grades 1-2 EGE, which allows one to recommend that this protein be identified as an additional criterion for predicting the effectiveness of IVF programs in order to expand indications for cryopreservation or delayed embryo transfer in more favorable conditions.
Keywords
infertility
endometriosis
in vitro fertilization
lactoferrin
α2-macroglobulin
albumin
tumor necrosis factor-α
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Received 13.01.2020
Accepted 07.02.2020
About the Authors
Victoria V. Likhacheva, PhD (medical science), teaching assistant of Department of obstetrics and gynecology in Novokuznetsk State Institute of Postgraduate Medicine.654005; Russia, Novokuznetsk, Stroiteley, 5.
Xenia V. Krasnopol’skaya, Corresponding Member, Russian Academy of Science, M.D. (Doctor of medical science), Professor, Head of Reproductive Medicine Dept.,
Moscow Regional Obstetrics and Gynecology Scientific Research Institute 101000; Russia, Moscow, Pokrovka st., 22.
Lyudmila G. Bazhenova, M.D. (Doctor of medical science), Professor, Head of Department of obstetrics and gynecology in Novokuznetsk State Institute of Postgraduate Medicine. 654005; Russia, Novokuznetsk, Stroiteley ave., 5.
Raisa M. Zorina, D.Sc. (Doctor of biological science), Principal Research fellow of Research Laboratory of Immunology in Novokuznetsk State Institute of Postgraduate Medicine. 654005; Russia, Novokuznetsk, Stroiteley ave., 5.
Arkadiy G. Markdorf, PhD (medical science), medical director of the Clinic «Medica» Group of Companies «Mother and Child» Novokuznetsk.
654041, Russia, Novokuznetsk, Sechenova, 30.
Lyudmila V. Renge, MD (Doctor of medical science) Professor of Department of obstetrics and gynecology in Novokuznetsk State Institute of Postgraduate Medicine Medicine. 654005; Russia, Novokuznetsk, Stroiteley ave, 5.
Tat’yana V. Tret’yakova, PhD (medical science), teaching assistant of Department of obstetrics and gynecology in Novokuznetsk State Institute of Postgraduate Medicine Medicine. 654005; Russia, Novokuznetsk, Stroiteley ave. 5.
Svetlana V. Shramko, PhD (medical science) Associate Professor of Teaching assistant of Department of obstetrics and gynecology in Novokuznetsk State Institute
for Further Training of Physicians – Branch Campus of the Federal State Budgetary Educational Institution of Further Professional Education «Russian Medical Academy
of Continuous Professional Education» of the Ministry of Healthcare of the Russian Federation.
654005; Russia, Novokuznetsk, Stroiteley ave. 5.
For citation: Likhacheva V.V., Krasnopolskaya K.V., Bazhenova L.G., Zorina R.M., Markdorf A.G., Renge L.V., Tretyakova T.V., Shramko S.V. Certain acute-phase proteins and cytokines in the serum and follicular liquid of infertile patients with grades 1-2 external genital endometriosis.
Akusherstvo i Ginekologiya/ Obstetrics and gynecology. 2020; 3: 100-9. (In Russian).
https://dx.doi.org/10.18565/aig.2020.3.100-109
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