Study of gene expressions in the eutopic endometrium of women with endometrioid ovarian cysts
Objective. To identify the genes, the expression of which in the eutopic proliferative endometrium differs in patients with endometrioid ovarian cysts (EOC) compared to that in the eutopic proliferative endometrium of women without endometriosis.Kuznetsova M.V., Pshenichnyuk E.Yu., Burmenskaya O.V., Asaturova A.V., Trofimov D.Yu., Adamyan L.V.
Subjects and methods. Two patient groups (10 women with EOC (a study group) and 10 women without endometriosis (a control group)) were examined. All the patients underwent a full clinical-anamnestic examination: history data collection, general physical and gynecological examinations, pelvic ultrasonography, clinical and laboratory examination. Eutopic endometrial tissue pipelle biopsy specimens were obtained directly intraoperatively before hysteroscopy, with subsequent endometrectomy and histological examination. Total RNA was isolated from the specimens; an expression profile was subsequently obtained by microarray hybridization using the GeneChip Human Exon 1.0 ST Arrays (Affymetrix, USA) according to the manufacturer’s protocol.
Results. The eutopic proliferative endometrium of the women with EOC (a study group) showed increased expression of 27 genes and decreased expression of 17 genes (the microarray measures the expression level of a total of 14318 transcripts) as compared with that from the women without endometriosis (a control group). All these genes are encountered in the works on eutopic endometrial expression; thus, our results are largely consistent with the published data, which makes these genes promising biomarkers for endometriosis.
Conclusion. The obtained data on the increased expression of the FOS, EGR-1, FOSB, DUSP1, ZFP36, JUNB, and JUN genes in the eutopic proliferative endometrium of women with EOC agree with the results of previously published works, which makes these genes promising biomarkers to create real-time PCR-based diagnostic systems for the determination of mRNA levels in endometrial tissues.
Keywords
endometriosis
eutopic endometrium
transcriptome
Human Exon 1.0
Supplementary Materials
- Table 1. Clinical characteristics of the studied population
- Table 2. Genes different expression in proliferative eutopic endometrium of women with ovarian endometrial cysts compared with proliferative eutopic endometrium of women without endometriosis
- Figure. Hierarchical cluster analysis eutopic endometrium from patients with ovarian endometrial cysts compared with proliferative eutopic endometrium of women without endometriosis (expression change ≥ 2,0)
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Received 16.06.2017
Acepted 23.06.2017
About the Authors
Maria Vladimirovna Kuznetsova, Ph.D, research scientist, Laboratory of molecular-genetic methods, Research Center of Obstetrics, Gynecology and Perinatology,Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381341. Е-mail: mkarja@mail.ru
Ekaterina Yuryevna Pshenichnyuk, PhD-student, Department of Surgical Gynecology, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79175591101. E-mail: best-kauty@mail.ru
Olga Vladimirovna Bourmenskaya, Ph.D., research scientist, Laboratory of molecular-genetic methods, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382292. E-mail: bourmenska@mail.ru
Alexandra Vyacheslavovna Asaturova, PhD, senior scientific researcher of pathology department, Research Center of Obstetrics, Gynecology and Perinatology,
Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954382311. E-mail: a_asaturova@oparina4.ru
Dmitry Yurievich Trofimov, DSc, Head of Laboratory of molecular-genetic methods, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954384951. Е-mail: d_trofimov@oparina4.ru
Leila Vagoevna Adamyan, MD, professor, academician of RAS; deputy director on science, head of department of operative gynecology, Research Center of Obstetrics, Gynecology and Perinatology, Ministry of Health of Russia. 119997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954387783. E-mail: l_adamyan@oparina4.ru
For citations: Kuznetsova M.V., Pshenichnyuk E.Yu., Burmenskaya O.V.,
Asaturova A.V., Trofimov D.Yu., Adamyan L.V. Study of gene expressions in the eutopic endometrium of women with endometrioid ovarian cysts.
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (8): 93-102. (in Russian)
http://dx.doi.org/10.18565/aig.2017.8.93-102
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