Chronic endometritis: The levels of regulatory and transport proteins in blood and intrauterine swabs in the prediction of the effectiveness of IVF programs
Objective. To estimate the levels of regulatory and transport proteins (alpha2-macroglobulin (α2-MG), lactoferrin (LF), and albumin (ALB)) in the blood and intrauterine swabs of patients with tubal-peritoneal infertility associated with chronic endometritis (CE) for the prediction of the effectiveness of IVF programs.Likhacheva V.V., Tretyakova Ya.N., Zorina V.N., Bazhenova L.G., Markdorf A.G., Sotnikova L.S., Zorin N.A.
Subjects and methods. The samples of blood and endometrial swabs were examined in 150 patients with tubal-peritoneal infertility associated with CE (a study group) and without chronic endometritis (a comparison group) who were treated to restore fertility by IVF in the „Medica” Clinic, “Mother and Child” Group of Companies (Novokuznetsk, Russia), in relation to the effectiveness of IVF programs before and after their implementation. Blood α2-MG and ALB levels were determined by quantitative rocket immunoelectrophoresis, the content of LF in blood and swabs and that of α2-MG and ALB in swabs were estimated by solid-phase enzyme immunoassay.
Results. The women with CE were found to have no considerable changes in the venous blood protein profile, regardless of the effectiveness of IVF programs. Local (intrauterine) antibacterial LF deficiency was found in women with infertility irrespective of its causes (CE or isolated tubal factor infertility). The subgroup of patients with ineffective IVF programs showed substantially elevated ALB and α2-MG levels in uterine swabs.
Conclusion. The found statistically significant changes suggest that there is a local reduction in the mucosal antibacterial potential in the presence of inflammatory manifestations and concomitant exudation of ALB and α2-MG from blood flow in women with tubal-peritoneal infertility associated with CE. This allows the author to recommend the determination of LF, ALB and α2-MG in uterine swabs in CE prior to the entry into the IVF program in order to predict its effectiveness.
Keywords
chronic endometritis
infertility
IVF
intrauterine swabs
lactoferrin
alpha2-macroglobulin
albumin
Supplementary Materials
References
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Received 01.07.2016
Accepted 02.09.2016
About the Authors
Likhacheva Victoria Vasilyevna, PhD (medical science), teaching assistant of Department of obstetrics and gynecology of Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia. 654005, Russia, Novokuznetsk, Stroiteley str. 5. Tel.: +79059053549. E-mail: viroli@mail.ruTretyakova Yana Nikolaevna, obstetrician-gynecologist, Perinatal Medical Center, “Mother and Child” Group of Companies.
654005, Russia, Moscow, Sevastopol Prospect 24 bld. 1. Tel.: +74953318510. E-mail: barly@yandex.ru
Zorina Veronika Nikolayevna, D.Sc. (Doctor of biological science), Principal Research fellow of Research Laboratory of Immunology of Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia. 654005, Russia, Novokuznetsk, Stroiteley str. 5. Tel.: +73843458418. E-mail: macroglobulin@yandex.ru
Bazhenova Lyudmila Grigoryevna, M.D. (Doctor of medical science), Professor, Head of Department of obstetrics and gynecology of Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia. 654005, Russia, Novokuznetsk, Stroiteley str. 5. Tel.: +73843796847. E-mail: l_bagenova@mail.ru
Markdorf Arkadiy Gennadyevich, PhD (medical science), medical director of the “Medica” Clinic, “Mother and Child” Group of Companies.
654041, Russia, Novokuznetsk, Sechenova str. 30. Tel.: +73843991919. E-mail: markdorf@mail.ru
Sotnikova Larisa Stepanovna, M.D. (Doctor of medical science), Professor of Department of obstetrics and gynecology in Siberian State Medical University,
Ministry of Health of Russia. 634050, Russia, Tomsk, Moscow highway 2. Tel.: +73822468700. E-mail: sotnikova-larisa@mail.ru
Zorin Nikolay Alexeevich, D.Sc. (Doctor of biological science), Professor, Head of Research Laboratory of Immunology of Novokuznetsk State Institute for Postgraduate Training of Physicians, Ministry of Health of Russia. 654005, Russia, Novokuznetsk, Stroiteley str. 5. Tel.: +73843458418. E-mail: macroglobulin@yandex.ru
For citations: Likhacheva V.V., Tretyakova Ya.N., Zorina V.N., Bazhenova L.G., Markdorf A.G., Sotnikova L.S., Zorin N.A. Chronic endometritis: The levels of regulatory and transport proteins in blood and intrauterine swabs in the prediction of the effectiveness of IVF programs. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (2): 58-62. (in Russian)
http://dx.doi.org/10.18565/aig.2017.2.58-62
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