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The Journal "Obstetrics and Gynecology"Journal HistoryAims and ScopePolicies on Conflict of Interest, Human and Animal Rights, and Informed ConsentEditorial ProcessData sharing statementAdvertising PolicyThe Process for Handling Cases Requiring Corrections, Retractions, and Editorial Expressions of ConcernEditorial BoardEditorial CounsilInformation for ProfessionalsNewsContacts
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Obstetrics and Gynegology2023№12
Application of fluorescent in situ...

Application of fluorescent in situ hybridization in the diagnosis of bacterial vaginosis

DOI
https://dx.doi.org/10.18565/aig.2023.129

Savicheva A.M., Krysanova A.A., Shalepo K.V., Spasibova E.V., Budilovskaya O.V., Khusnutdinova T.A., Tapilskaya N.I., Kogan I.Yu., Swidsinski A.V., Swidsinski S.

1) D.O. Ott Research Institute of Obstetrics, Gynecology and Reproduction, St. Petersburg, Russia; 2) St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, St. Petersburg, Russia; 3) St. Petersburg State University, St. Petersburg, Russia; 4) Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charit, Hospital, Berlin, Germany; 5) International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms; 6) I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia, Moscow, Russia

Bacterial vaginosis (BV) is a polymicrobial biofilm vaginal syndrome characterized by a high prevalence, recurrence rate and associated complications, including preterm birth, infertility and a higher risk of sexually transmitted infections. Traditional diagnostic methods used to detect the disease do not provide complete information on the morphology, number, and spatial arrangement of microorganisms associated with BV. Fluorescence in situ hybridization (FISH) combines the accuracy of molecular genetics with the informative power of microscopy to visualize the relationships between bacteria in their natural microhabitat, such as the biofilm in BV. The persistence of biofilm composed of BV-associated microorganisms is one of the most likely pathways for recurrence and is an important diagnostic marker for the disease.  The review of the literature presents the history of the use of FISH, outlines its basic principles and demonstrates its advantages in the diagnosis of bacterial vaginosis, especially its recurrent forms.
Conclusion: The use of the FISH method may not only change the understanding of BV pathogenesis, but it can also identify the etiological agent in each particular case, diagnose biofilm/non-biofilm vaginosis by determining the spatial relationship of bacteria to each other and to epithelial cells, predict the recurrence of the disease and choose the appropriate therapy.

Authors’ contributions: Savicheva A.M., Krysanova A.A. – review of publications on the topic of the article, analysis of the data obtained, writing of the manuscript, approval of the final version of the article; Shalepo K.V., Spasibova E.V., Budilovskaya O.V., Khusnutdinova T.A., Tapilskaya N.I., Swidsinski S. – manuscript editing, approval of the final version of the article; Kogan I.Yu.,  Swidsinski A.V. – final manuscript editing and approval of the final version of the article. 
Conflicts of interest: The authors have no conflicts of interest to declare. 
Funding: The study was conducted within the framework of the initiative theme. 
For citation: Savicheva A.M., Krysanova A.A., Shalepo K.V., Spasibova E.V., Budilovskaya O.V., Khusnutdinova T.A., Tapilskaya N.I., Kogan I.Yu., Swidsinski A.V., Swidsinski S. Application of fluorescent in situ hybridization in the diagnosis of bacterial vaginosis. 
Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2023; (12): 68-77 (in Russian)
https://dx.doi.org/10.18565/aig.2023.129

Keywords

recurrent bacterial vaginosis recurrent
biofilm
FISH
Gardnerella spp
Full text (in Russian)

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Received 22.05.2023

Accepted 20.09.2023

About the Authors

Alevtina M. Savicheva, Dr. Med. Sci., Professor, Head of the Department of Medical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russiua, St. Petersburg, Mendeleevskaya line, 3; Head of the Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2; Head of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms, savitcheva@mail.ru, https://orcid.org/0000-0003-3870-5930
Anna A. Krysanova, PhD, MD, Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia,
St. Petersburg, Mendeleyevskaya line, 3; Assistant, Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2; PhD, MD, Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms, krusanova.anna@mail.ru, https://orcid.org/0000-0003-4798-1881
Kira V. Shalepo, PhD, MD, Senior Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, St. Petersburg, Mendeleevskaya line, 3; Associate Professor, Department of Clinical Laboratory Diagnostics of AF and DPO, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2; PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms, 2474151@mail.ru, https://orcid.org/0000-0002-3002-3874
Elena V. Spasibova, bacteriologist, Laboratory of Clinical Microbiology, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, St. Petersburg, Mendeleyevskaya line, 3; Assistant, Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2; bacteriologist, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms, elena.graciosae@gmail.com, https://orcid.org/0009-0002-6070-4651
Olga V. Budilovskaya, PhD, MD, Senior Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleevskaya line, 3; Assistant, Department of Clinical Laboratory Diagnostics of AF and DPO, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2; PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms, o.budilovskaya@gmail.com, https://orcid.org/0000-0001-7673-6274
Tatiana A. Khusnutdinova, PhD, MD, Senior Researcher, Experimental Microbiology Group, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, St. Petersburg, Mendeleyevskaya line, 3; Assistant, Department of Clinical Laboratory Diagnostics, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2; PhD, MD, Senior Researcher, International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms, husnutdinovat@yandex.ru, https://orcid.org/0000-0002-2742-2655
Natalya I. Tapilskaya, Dr. Med. Sci., Professor, Leading Researcher of the Reproduction Department, D.O. Ott Research Institute of Obstetrics, Gynecology and Reproductology, 199034, Russia, St. Petersburg, Mendeleevskaya line, 3; Professor, Department of Obstetrics and Ginecology, St. Petersburg State Pediatric Medical University, Ministry of Health of Russia, 194100, Russia, St. Petersburg, Litovskaya str., 2, tapnatalia@ yandex.ru, https://orcid.org/0000-0001-5309-0087
Igor Yu. Kogan, Corresponding Member of the RAS, Dr. Med. Sci., Professor, Director, D.O. Ott Reasearch Institute of Obstetrics, Gynecology and Reproductology,
199034, Russia, St. Petersburg, Mendeleyevskaya line, 3; Professor, St. Petersburg State University, St. Petersburg, Russia, ovr@ott.ru, https://orcid.org/0000-0002-7351-6900
Alexander V. Swidsinski, Head of the Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charité CCM, Medizinische Klinik, Universitätsmedizin,
10117 Berlin, Germany; Co-Head of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms,
alexander.swidsinski@charite.de, https://orcid.org/0000-0002-7071-0417
Sonia Swidsinski, bacteriologist of the Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charité CCM, Medizinische Klinik, Universitätsmedizin,
10117 Berlin, Germany; bacteriologist of the International Center for the Study of the Vitality and Resistance of Polymicrobial Communities and Biofilms,
alexander.swidsinski@charite.de
Corresponding author: Anna A. Krysanova, krusanova.anna@mail.ru
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