Search for personalized implantation window markers in women in an in vitro fertilization program by identifying the transcriptional profile of genes
Objective. To enhance the efficiency of determining the endometrial functional status during the implantation window based on the integrated assessment of gene transcripts as markers of endometrial readiness for embryo implantation.Burmenskaya O.V., Bozhenko V.K., Smolnikova V.Yu., Kalinina E.A., Korneeva I.E., Mezhevitinova E.A., Donnikov A.E., Beik E.P., Nabieva K.R., Naumov V.A., Borovikov P.I.
Subjects and methods. The investigation enrolled 71 women with realized reproductive function without endometrial disease; of them 47 patients did this after in vitro fertilization and embryo transfer. During the expected implantation window, pipelle endometrial biopsy in a natural cycle and histological examination were performed and the transcriptional profile of the GPX3, PAEP, DPP4, TAGLN, HABP2, IMPA2, AQP3, HLA-DOB, MSX1, and POSTN genes was determined by real-time quantitative reverse transcription polymerase chain reaction.
Results. There were differences in the level of mRNA expression of all the examined genes in the receptive endometrium versus prereceptive one, which could classify two endometrial functional states. An integrated assessment of the mRNA expression of 4 genes (PAEP, DPP4, MSX1 and HLA-DOB) made it possible to determine endometrial receptivity. This was consistent with histological findings of the stage of endometrial maturation in 83% of cases.
Conclusion. A model has been proposed to determine a personalized implantation window, which can be further used in assisted reproductive technology programs.
Keywords
References
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Received 03.11.2016
Accepted 11.11.2016
About the Authors
Burmenskaya Olga Vladimirovna, Ph.D., D.Sc., Senior Researsher of molecular-genetics Department, Research Center of Obstetrics, Gynecology, and Perinatology,Ministry of Health of Russia. 117997, Russia, Moscow, Oparina str. 4. Tel.: +74954382292. E-mail: o_bourmenskaya@oparina4.ru
Bozhenko Vladimir Konstantinovich, Ph.D., M.D., Professor, Consultant, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Oparina str. 4. Tel.: +74991201114. E-mail: vbojenko@mail.ru
Smolnikova Veronika Yurievna, M.D., Ph.D., Leading Researcher of the Department of assistive reproductive technology in the treatment of infertility,
Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Oparina str. 4. Tel.:+74954381341. E-mail: v_smolnikova@oparina4.ru
Kalinina Elena Anatolievna, M.D., Ph.D., Head of the Department of assistive reproductive technology in the treatment of infertility, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia. 117997, Russia, Moscow, Oparina str. 4. Tel.: +74954381341. E-mail: e_kalinina@oparina4.ru
Korneeva Irina Evgenievna, M.D., Ph.D., Leading Researcher of the 1 gynecology department, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Oparina str. 4. Tel.: +74954382622. E-mail: i_korneeva@oparina4.ru
Mezhevitinova Elena Anatolievna, M.D., Ph.D., Leading Researcher of the scientific-patient Department, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Oparina str. 4. Tel.: +74954381403. E-mail: e_mezhevitinova@oparina4.ru
Donnikov Andrey Evgenyevich, Ph.D., senior staff scientist of molecular-genetics Department, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Oparina str. 4. Tel.: +74954384951. E-mail: a_donnikov@oparina4.ru
Beyk Ekaterina Pavlovna, graduate student, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Oparina str. 4. Tel.: +79050343333. E-mail: e_beyk@oparina4.ru
Nabieva Kemalya Ragimovna, graduate student Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Oparina str. 4. Tel.: +79255679926. E-mail: kemalya-n@yandex.ru
Naumov Vladimir Aleksandrovich, Scientific Researsher of laboratory bioinformatics, Research Center of Obstetrics, Gynecology, and Perinatology,
Ministry of Health of Russia. 117997, Russia, Moscow, Oparina str. 4. Tel.: +74954384951. E-mail: v_naumov@oparina4.ru
Borovikov Pavel Igorevich, Head of laboratory bioinformatics, Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of Russia.
117997, Russia, Moscow, Oparina str. 4. Tel.: +74954384951. E-mail: p_borovikov@oparina4.ru
For citations: Burmenskaya O.V., Bozhenko V.K., Smolnikova V.Yu., Kalinina E.A.,
Korneeva I.E., Mezhevitinova E.A., Donnikov A.E., Beik E.P., Nabieva K.R., Naumov V.A., Borovikov P.I. Search for personalized implantation window markers in women in an in vitro fertilization program by identifying the transcriptional profile of genes. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (5): 72-80. (in Russian)
http://dx.doi.org/10.18565/aig.2017.5.72-80