Features of the composition of peripheral blood lymphocyte subpopulations with cytotoxic, killer, and regulatory functions in women with recurrent implantation failures in ART programs
Objective. To analyze the content of peripheral blood lymphocyte subpopulations with cytotoxic, killer, and regulatory functions in female patients with recurrent implantation failures in assisted reproductive technology programs.Amyan T.S., Krechetova L.V., Perminova S.G., Vtorushina V.V.
Subjects and methods. The investigation enrolled 48 patients with recurrent implantation failures in an IVF program and 26 fertile women with their last child aged less than 2 years.
Results. There was a reduction in T lymphocytes (CD3+) and T lymphocytes with cytotoxic function (CD3+CD8+) and an increase in innate immunity cells with both cytotoxic (CD3-CD8+) and killer (CD56+, CD16+, CD56,16+) functions. The group patients showed a significant rise in gd+Т cell levels in a cryo-cycle (p = 0.005).
Conclusion. In patients with recurrent implantation failures, the composition of lymphocyte subpopulations is characterized by a decrease in T lymphocytes (CD3+) and T lymphocytes with cytotoxic function (CD3+CD8+) and by an increase in innate immunity cells with both cytotoxic and killer functions.
Keywords
recurrent implantation failures
CD56+
CD16+
CD3-CD8+
CD3+CD8+
gd+Т cells
Supplementary Materials
- Table 1- The content of different phenotypes of lymphocytes in the peripheral blood of patients with repeated implantation failure and in fertile women (M±m)
- Table 2- The content of different phenotypes of lymphocytes in the peripheral blood of patients in the patients in IVF cycles and frozen/thawed embryo transfer cycles (M±m)
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Received 08.09.2017
Accepted 22.09.2017
About the Authors
Amyan Tatiana S., a graduate student of the 1st year of study department of conservation and restoration of reproductive function, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79261632833.E-mail: amyantanya@rambler.ru
Krechetova Lubov V., PhD, Senior Research Fellow, Head of the Laboratory Laboratory of Clinical Immunology, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: k_l_v_@mail.ru
Perminova Svetlana G., MD, a leading researcher department of conservation and restoration of reproductive function, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +79162021687. E-mail: perisvet@list.ru
Vtorushina Valentina V., PhD, doctor of clinical laboratory diagnostics, National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov, Ministry of Health of Russia. 117997, Russia, Moscow, Ac. Oparina str. 4. Tel.: +74954381183. E-mail: vtorushina@inbox.ru
For citations: Amyan T.S., Krechetova L.V., Perminova S.G., Vtorushina V.V. Features of the composition of peripheral blood lymphocyte subpopulations with cytotoxic, killer, and regulatory functions in women with recurrent implantation failures in ART programs. Akusherstvo i Ginekologiya/Obstetrics and Gynecology. 2017; (12): 78-83. (in Russian)
https://dx.doi.org/10.18565/aig.2017.12.78-83
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